Diabetes Care, Vol 17, Issue 1 57-63, Copyright © 1994 by American Diabetes Association

ARTICLES

Decreased polymorphonuclear leukocyte deformability in NIDDM

Z Pecsvarady, TC Fisher, CH Darwin, A Fabok, TS Maqueda, MF Saad and HJ Meiselman
Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033.

OBJECTIVE--To determine the rheological properties of polymorphonuclear leukocytes (PMN) from non-insulin-dependent diabetes mellitus (NIDDM) patients. RESEARCH DESIGN AND METHODS--The deformability of PMN from 33 NIDDM subjects, 13 with impaired glucose tolerance (IGT), and 22 with normal glucose tolerance (NGT) was studied. A Cell Transit Analyzer that measures the transit time of PMN through 8-microns pores was used. Studies were performed under three different conditions: 1) basal state; 2) after incubation with cytochalasin B (20 microM) to dissociate f-actin from the cytoskeleton; and 3) following activation with N-formyl-methionyl-leucyl-phenylalanine (fMLP, 1 nM). RESULTS--PMN from diabetic patients were more rigid (i.e., had longer transit time) than those from subjects with NGT or IGT under basal conditions and after cytochalasin B, but not after stimulation with fMLP. The deformability of PMN from subjects with IGT was similar to those of the NGT group. In the pooled data, basal transit time correlated with age; systolic and diastolic blood pressure; HbA1c; and serum creatinine, cholesterol, and triglyceride concentrations (r = 0.29, 0.34, 0.37, 0.48, 0.25, 0.36, 0.29, respectively, P < 0.05 for each). Hypertensive diabetic patients had less deformable PMN than normotensive ones. No relation was found between PMN deformability and the duration of diabetes, type of treatment, or the presence of retinopathy. CONCLUSIONS--These data indicate increased rigidity of PMN in NIDDM that may contribute to development of microcirculatory disturbances and microangiopathy.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Y. Kim, M. A. Johnson, D. S. McLeod, T. Alexander, B. C. Hansen, and G. A. Lutty Neutrophils Are Associated With Capillary Closure in Spontaneously Diabetic Monkey Retinas Diabetes, May 1, 2005; 54(5): 1534 - 1542. [Abstract] [Full Text] [PDF]

				J R MacKinnon, R M Knott, and J V Forrester Altered L-selectin expression in lymphocytes and increased adhesion to endothelium in patients with diabetic retinopathy Br. J. Ophthalmol., September 1, 2004; 88(9): 1137 - 1141. [Abstract] [Full Text] [PDF]

				D. T. Goldenberg, G. N. Holland, W. G. Cumberland, T. C. Fisher, I.-C. Folz, R. C. Wang, B. G. Terry, A. A. Moe, F. Kramer, J. I. Lim, et al. An Assessment of Polymorphonuclear Leukocyte Rigidity in HIV-Infected Individuals after Immune Recovery Invest. Ophthalmol. Vis. Sci., June 1, 2002; 43(6): 1857 - 1861. [Abstract] [Full Text] [PDF]

				A P Adamis Is diabetic retinopathy an inflammatory disease? Br. J. Ophthalmol., April 1, 2002; 86(4): 363 - 365. [Full Text] [PDF]

				G B ARDEN The absence of diabetic retinopathy in patients with retinitis pigmentosa: implications for pathophysiology and possible treatment Br. J. Ophthalmol., March 1, 2001; 85(3): 366 - 370. [Full Text]

				A. Tufail, G. N Holland, T. C Fisher, W. G Cumberland, and H. J Meiselman Increased polymorphonuclear leucocyte rigidity in HIV infected individuals Br. J. Ophthalmol., July 1, 2000; 84(7): 727 - 731. [Abstract] [Full Text]

				E. M. DROST, G. KASSABIAN, H. J. MEISELMAN, D. GELMONT, and T. C. FISHER Increased Rigidity and Priming of Polymorphonuclear Leukocytes in Sepsis Am. J. Respir. Crit. Care Med., June 1, 1999; 159(6): 1696 - 1702. [Abstract] [Full Text]

				S. U. Persson, H. Larsson, and H. Odeberg Reduced Number of Circulating Monocytes After Institution of Insulin Therapy--Relevance for Development of Atherosclerosis in Diabetics? Angiology, June 1, 1998; 49(6): 423 - 433. [Abstract] [PDF]