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Diabetes Care, Vol 22, Issue 2 275-279, Copyright © 1999 by American Diabetes Association

ARTICLES

Capillary whole blood measurement of islet autoantibodies

E Bazzigaluppi, R Bonfanti, PJ Bingley, E Bosi and E Bonifacio
Department of Medicine, Istituto Scientifico San Raffaele, Milan, Italy.

OBJECTIVE: Islet cell antibody (ICA) measurements in serum are used for large-scale screening to identify subjects who are at high risk of developing type 1 diabetes. The aim of this study was to adapt measurements to capillary whole blood samples to facilitate and reduce screening costs. RESEARCH DESIGN AND METHODS: GAD65, IA-2, and combined GAD65/IA-2 antibody tests were performed on patients with type 1 diabetes, first-degree relatives of patients, and control subjects, and results from serum, plasma, whole venous blood, and capillary whole blood lysates were compared. Measurements obtained in serum and eluates from dried capillary blood spots from 36 ICA+ first-degree relatives were also compared. RESULTS: GAD65, IA-2, and combined GAD65/IA-2 antibody levels were completely concordant with measurements obtained from serum, plasma, whole venous blood, and capillary whole blood lysates. Antibody levels obtained in eluates from dried capillary blood spots were lower than corresponding serum samples, and weak antibodies were not detected. CONCLUSIONS: Initial screening for diabetes risk can be performed using one drop of capillary whole blood without further processing to separate serum. This method should be considered as a way to simplify and reduce costs of screening programs.
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 J. Clin. Endocrinol. Metab.Home page
M.-R. Pastore, E. Bazzigaluppi, C. Belloni, C. Arcovio, E. Bonifacio, and E. Bosi
Six Months of Gluten-Free Diet Do Not Influence Autoantibody Titers, but Improve Insulin Secretion in Subjects at High Risk for Type 1 Diabetes
J. Clin. Endocrinol. Metab., January 1, 2003; 88(1): 162 - 165.
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Copyright © 1999 by the American Diabetes Association.