HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Matsunaga, H. Articles by Kawamori, R. Search for Related Content PubMed PubMed Citation Articles by Matsunaga, H. Articles by Kawamori, R. Social Bookmarking                What's this?

Antiatherogenic Mitochondrial Genotype in Patients With Type 2 Diabetes

¹ Department of Medicine, Metabolism and Endocrinology, Juntendo University, Tokyo; and the
² Department of Gene Therapy, Gifu International Institute of Biotechnology, Gifu, Japan

OBJECTIVE—To evaluate the significance of a longevity-associated mitochondrial genotype (Mt5178A) derived from a C A transversion at nucleotide position 5178 of mitochondrial DNA, which causes a Leu-to-Met substitution within the NADH dehydrogenase subunit 2 gene, in type 2 diabetic subjects.

RESEARCH DESIGN AND METHODS—Mt5178 typing was done by polymerase chain reaction–restriction fragment-length polymorphism with the restriction enzyme AluI in 1,148 type 2 diabetic Japanese subjects, and the results were compared with the clinical characteristics. Then, the association of Mt5178 type with early atherosclerotic changes of the bilateral carotid arteries on ultrasonography was assessed in 412 diabetic subjects randomly selected from the original 1,148 type 2 diabetic subjects, while maintaining the same frequency of Mt5178A and Mt5178C.

RESULTS—The frequency of Mt5178A in the type 2 diabetic subjects (454 of 1,148; 40%) was not different from that previously found in healthy blood donors (114 of 252; 45%). Clinical characteristics regarding diabetes were not significantly different between the Mt5178A group (n = 454) and the Mt5178C group (n = 694). However, the mean intima-media thickness (IMT) at six sites in the bilateral carotid arteries was significantly smaller in the Mt5178A group than in the Mt5178C group (0.906 ± 0.018 vs. 0.995 ± 0.021 mm, mean ± SEM, P = 0.022), and the Mt5178 type was significantly correlated with both the mean IMT and the presence of plaque on multiple regression analysis and discriminant analysis.

CONCLUSIONS—The Mt5178A genotype may be unrelated to the etiology of type 2 diabetes. However, Mt5178A seems to have an antiatherogenic effect, at least in type 2 diabetic individuals.

Abbreviations: CVD, cerebrovascular disease • IHD, ischemic heart disease • IMT, intima-media thickness • mtDNA, mitochondrial DNA • ND2, NADH dehydrogenase subunit 2 • PCR, polymerase chain reaction • ROS, reactive oxygen species

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. M. Gusdon, T. V. Votyakova, and C. E. Mathews mt-Nd2a Suppresses Reactive Oxygen Species Production by Mitochondrial Complexes I and III J. Biol. Chem., April 18, 2008; 283(16): 10690 - 10697. [Abstract] [Full Text] [PDF]

				A. M. Gusdon, T. V. Votyakova, I. J. Reynolds, and C. E. Mathews Nuclear and Mitochondrial Interaction Involving mt-Nd2 Leads to Increased Mitochondrial Reactive Oxygen Species Production J. Biol. Chem., February 23, 2007; 282(8): 5171 - 5179. [Abstract] [Full Text] [PDF]

				T. Maeno, H. Koyama, H. Tahara, M. Komatsu, M. Emoto, T. Shoji, M. Inaba, T. Miki, Y. Okuno, and Y. Nishizawa The 807T Allele in {alpha}2 Integrin Is Protective Against Atherosclerotic Arterial Wall Thickening and the Occurrence of Plaque in Patients With Type 2 Diabetes Diabetes, May 1, 2002; 51(5): 1523 - 1528. [Abstract] [Full Text] [PDF]