Diabetes Care
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Geerlings, S. E.
Right arrow Articles by Hoepelman, A. I.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Geerlings, S. E.
Right arrow Articles by Hoepelman, A. I.M.
Social Bookmarking
Add to CiteULike   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Diabetes Care 25:1405-1409, 2002
© 2002 by the American Diabetes Association, Inc.

Pathophysiology/Complications
Original Article

Adherence of Type 1-Fimbriated Escherichia coli to Uroepithelial Cells

More in diabetic women than in control subjects

Suzanne E. Geerlings, MD, PHD1, Ruby Meiland, MD2, Emiel C. van Lith, MD2, Ellen C. Brouwer3, Wim Gaastra, PHD4 and Andy I.M. Hoepelman, MD, PHD2,3

1 Department of Internal Medicine, Vrije Universiteit Medical Center, Amsterdam, the Netherlands
2 Department of Acute Medicine and Infectious Diseases, University Medical Center Utrecht, Utrecht, the Netherlands
3 Eijkman-Winkler Laboratory for Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands
4 Department of Bacteriology, Institute of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Utrecht, the Netherlands

OBJECTIVE—Women with diabetes have bacteriuria more often than women without diabetes. Because Escherichia coli adhere better to vaginal cells of nondiabetic patients with recurrent urinary tract infections (UTIs) than to those obtained from healthy control subjects, it was hypothesized that E. coli adhere more to the uroepithelial cells of diabetic women, either because of substances excreted in the urine (e.g., albumin, glucose, and Tamm Horsfall protein) or because of a difference in the uroepithelial cells.

RESEARCH DESIGN AND METHODS—A T24 bladder cell line and uroepithelial cells of 25 diabetic women and 19 control subjects were incubated with three different E. coli strains.

RESULTS—The mean numbers of type 1-fimbriated E. coli that adhered to diabetic and control cells were 12.9 and 6.1 (P = 0.001), respectively, whereas those of P-fimbriated E. coli were 8.8 and 8.1 (P = 0.8), and those of nonfimbriated E. coli were 2.7 and 3.4 (P = 0.4). The addition of various substances did not influence the adherence of E. coli to a T24 bladder cell line.

CONCLUSIONS—Type 1-fimbriated E. coli adhere more to diabetic than to control uroepithelial cells.

Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium • IMDM, Isocove’s modified Dulbecco’s medium • MSHA, mannose-sensitive hemagglutination • MRHA, mannose-resistant hemagglutination • THP, Tamm Horsfall protein • UTI, urinary tract infection


Add to CiteULike CiteULike   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
D. A. Rosen, C.-S. Hung, K. A. Kline, and S. J. Hultgren
Streptozocin-Induced Diabetic Mouse Model of Urinary Tract Infection
Infect. Immun., September 1, 2008; 76(9): 4290 - 4298.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
R. Meiland, S. E. Geerlings, R. P. Stolk, P. M. Netten, P. M. Schneeberger, and A. I. M. Hoepelman
Asymptomatic Bacteriuria in Women With Diabetes Mellitus: Effect on Renal Function After 6 Years of Follow-up.
Arch Intern Med, November 13, 2006; 166(20): 2222 - 2227.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Xie, G. Zhou, S.-Y. Chan, E. Shapiro, X.-P. Kong, X.-R. Wu, T.-T. Sun, and C. E. Costello
Distinct Glycan Structures of Uroplakins Ia and Ib: STRUCTURAL BASIS FOR THE SELECTIVE BINDING OF FimH ADHESIN TO UROPLAKIN Ia
J. Biol. Chem., May 26, 2006; 281(21): 14644 - 14653.
[Abstract] [Full Text] [PDF]

Home page
 Obstet GynecolHome page
J. S. Sheffield and F. G. Cunningham
Urinary Tract Infection in Women
Obstet. Gynecol., November 1, 2005; 106(5): 1085 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
B J Philips, J Redman, A Brennan, D Wood, R Holliman, D Baines, and E H Baker
Glucose in bronchial aspirates increases the risk of respiratory MRSA in intubated patients
Thorax, September 1, 2005; 60(9): 761 - 764.
[Abstract] [Full Text] [PDF]

Home page
 ANN INTERN MEDHome page
D. Scholes, T. M. Hooton, P. L. Roberts, K. Gupta, A. E. Stapleton, and W. E. Stamm
Risk Factors Associated with Acute Pyelonephritis in Healthy Women
Ann Intern Med, January 4, 2005; 142(1): 20 - 27.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
J. S. Brown, H. Wessells, M. B. Chancellor, S. S. Howards, W. E. Stamm, A. E. Stapleton, W. D. Steers, S. K. Van Den Eeden, and K. T. McVary
Urologic Complications of Diabetes
Diabetes Care, January 1, 2005; 28(1): 177 - 185.
[Full Text] [PDF]

Home page
 The Annals of PharmacotherapyHome page
S.-T. Ooi, L. A Frazee, and W. G Gardner
Management of Asymptomatic Bacteriuria in Patients with Diabetes Mellitus
Ann. Pharmacother., March 1, 2004; 38(3): 490 - 493.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Diabetes Diabetes Care Clinical Diabetes Diabetes Spectrum
Copyright © 2002 by the American Diabetes Association.