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Diabetes Care 27:2501-2502, 2004
© 2004 by the American Diabetes Association, Inc.

Brief Report

White Blood Cells in Obesity and Diabetes

Effects of weight loss and normalization of glucose metabolism

Anna Veronelli, MD1, Marco Laneri, MD1, Roberto Ranieri, MD1, Diana Koprivec, MD1, Debora Vardaro, BSC2, Michele Paganelli, MD3, Franco Folli, MD2 and Antonio E. Pontiroli, MD1

1 Cattedra di Medicina Interna, Dipartimento di Medicina, Chirurgia e Odontoiatria, Università Degli Studi di Milano, Milano: Divisione di Medicina Interna, Ospedale San Paolo, Milano, Italy
2 Divisione di Medicina Interna, Ospedale San Raffaele, Milano, Italy
3 Divisione di Chirurgia, Ospedale San Raffaele, Milano, Italy

Address correspondence and reprint requests to Anna Veronelli, Ospedale San Paolo, Via A di Rudinì 8, 20142 Milano, Italy. E-mail: annaveronelli{at}aliceposta.it

Abbreviations: HOMA, homeostasis model assessment • IGT, impaired glucose tolerance • LAGB, laparoscopic adjustable gastric banding • WBC, white blood cell


   INTRODUCTION
TOP
 INTRODUCTION
RESEARCH DESIGN AND METHODS
 RESULTS
 CONCLUSIONS
 References
 
White blood cell (WBC) count is elevated in obesity (1) and is a risk factor for atherosclerosis (2). An elevated WBC count is present in impaired glucose tolerance (IGT) (3), and WBC count is associated with macro- and microangiopathic complications in type 2 diabetes (4). In both of these studies, the effect was more marked in obese patients. In morbid obesity, bariatric surgery in many cases (gastric bypass or laparoscopic adjustable gastric banding [LAGB]) is able to induce, together with a sustained and durable weight loss, the disappearance of comorbidities, such as type 2 diabetes (5,6). The aim of this study was to compare the effect of LAGB and of conventional diet on WBC count in patients with morbid obesity that is simple or complicated by IGT or type 2 diabetes.


   RESEARCH DESIGN AND METHODS
TOP
 INTRODUCTION
 RESEARCH DESIGN AND METHODS
RESULTS
 CONCLUSIONS
 References
 
According to the protocol approved by the local ethics committee, all patients underwent a full diagnostic evaluation completed by psychological-psychiatric assessment (6). Patients who were suitable for surgery at the end of the diagnostic workup, but refused LAGB for personal reasons, underwent conventional treatment. Patients were studied under basal conditions and after 1–3 years of follow-up. Table 1 shows the clinical and laboratory variables of patients. Laboratory methods used in this study have already been published (6,7). For each variable, differences between basal conditions and follow-up were assessed using the Student’s t test for paired data. Pairwise regression analysis between change in WBC count and change in clinical and metabolic variables was calculated. Stepwise regression analysis was further carried out to estimate the independent contribution of selected variables (variables significant at linear regression plus age and sex) on change in WBC count. P values <0.05 were considered statistically significant.


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  		Table 1— Clinical and metabolic variables of subjects in the study

 

   RESULTS
TOP
 INTRODUCTION
 RESEARCH DESIGN AND METHODS
 RESULTS
CONCLUSIONS
 References
 
Table 1 shows that under basal conditions the two groups were not different as to clinical and metabolic variables. At follow-up, BMI, fasting blood glucose and insulin, homeostasis model assessment (HOMA) index, and WBC count decreased significantly with LAGB, not in the other group. HbA1c decreased significantly in both groups. Glucose tolerance improved with LAGB, not in the other group. Change in WBC count correlated with change in BMI (r = 0.342, P = 0.0016), in insulin (r = 0.278, P = 0.0126), and in HOMA (r = 0.283, P = 0.0132) but not with change in glucose tolerance. At stepwise regression analysis (age, sex, change in BMI, and change in HOMA [or change in insulin] were the variables in the model), BMI change was the only predictor of WBC count change (P = 0.01).


   CONCLUSIONS
TOP
 INTRODUCTION
 RESEARCH DESIGN AND METHODS
 RESULTS
 CONCLUSIONS
References
 
These data indicate the importance of weight loss in reducing WBC count in morbid obesity, simple or complicated by IGT or type 2 diabetes. This finding agrees with a previous report indicating that adhesion molecules (intercellular adhesion molecule-1 and E-selectin) decrease in morbidly obese patients after significant weight loss, not after normalization of glucose metabolism (7).

The mechanism through which weight loss is associated with a decrease in WBC count is a matter of speculation, and one hypothesis is that leptin might be involved in the decrease in WBC count. In fact, leptin stimulates myeloid differentiation (8) and decreases after weight loss (9). On the other hand, insulin decreases after weight loss as well, but the available evidence indicates that insulin has an antiapoptotic effect only on myocytes (10). IGF-1 has an antiapoptotic effect on WBC count (11), but IGF-1 levels are not increased in obese subjects and do not change after weight loss (12).

Another aspect of interest is the potential value of a decrease of 1,000 WBCs/µl (–11%) within the normal range. According to Tong et al. (2), an increase of 1,000 WBCs/µl is associated with an increased 15.8% risk of macrovascular complications and with an increased 12.3% risk of microvascular complications. Cavalot et al. (13) have shown a linear regression between WBC count, within the normal range, and albumin excretion rate. Leukocytes participate in the inflammation process, are recruited at the site of endothelial injury, and form foam cells in the plaque (14). Interleukins and tumor necrosis factor-{alpha} are released from activated leukocytes and cause endothelial dysfunction (15). In line with these data, sustained weight loss is accompanied by a decrease in interleukins and in tumor necrosis factor-{alpha} and by improvement of endothelial dysfunction (16).

A longer follow-up is required to verify whether sustained and long-lasting weight loss decreases the risk for macrovascular complications in obesity and diabetes.


   Acknowledgments
 
This research was supported by Grant FIRST 2002 from the Università degli Studi di Milano, from the Ministero dell’Università e della Ricerca Scientifica e Tecnologica 2002 (grant 2002064582_003), from the Ministero della Salute (grant 199/02) (to A.E.P.), and from the Ministero della Salute (to F.F.).

This work was presented in abstract form at the 64th Annual Meeting of the American Diabetes Association, Orlando, Florida, 4–8 June 2004.


   Footnotes
 
A table elsewhere in this issue shows conventional and Système International (SI) units and conversion factors for many substances.

Received for publication June 8, 2004. Accepted for publication June 23, 2004.


   References
TOP
 INTRODUCTION
 RESEARCH DESIGN AND METHODS
 RESULTS
 CONCLUSIONS
 References
 

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  2. Elkind MS, Cheng J, Boden-Albala B, Paik MC, Sacco RL, Northern Manhattan Stroke Study: Elevated white blood cell count and carotid plaque thickness: the Northern Manhattan Stroke study. Stroke 32:842–849, 2001[Abstract/Free Full Text]
  3. Ohshita K, Yamane K, Hanafusa M, Mori H, Mito K, Okubo M, Hara H, Kohno N: Elevated white blood cell count in subjects with impaired glucose tolerance. Diabetes Care 27:491–496, 2004[Abstract/Free Full Text]
  4. Tong PC, Lee KF, So WY, Ng MH, Chan WB, Lo MK, Chan NN, Chan JC: White blood cell count is associated with macro- and microvascular complications in Chinese patients with type 2 diabetes. Diabetes Care 27:216–222, 2004[Abstract/Free Full Text]
  5. Pories WJ, Swanson MS, MacDonald KG, Long SB, Morris PG, Brown BM, Barakat HA, deRamon RA, Israel G, Dolezal JM: Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus. Ann Surg 222:339–350, 1995[Medline]
  6. Pontiroli AE, Pizzocri P, Librenti MC, Vedani P, Marchi M, Cucchi E, Orena C, Paganelli M, Giacomelli M, Ferla G, Folli F: Laparoscopic adjustable gastric banding for the treatment of morbid (grade 3) obesity and its metabolic complications: a three-year study. J Clin Endocrinol Metab 87:3555–3561, 2002[Abstract/Free Full Text]
  7. Pontiroli AE, Pizzocri P, Koprivec D, Vedani P, Marchi M, Arcelloni C, Paroni R, Esposito K, Giugliano D: Body weight and glucose metabolism have a different effect on circulating levels of ICAM-1, E-selectin, and endothelin-1 in humans. Eur J Endocrinol 150:195–200, 2004[Abstract]
  8. Laharrague P, Oppert JM, Brousset P, Charlet JP, Campfield A, Fontanilles AM, Guy-Grand B, Corberand JX, Penicaud L, Casteilla L: Leptin stimulates myeloid differentiation from human bone marrow CD34+ progenitors: potential involvement in leukocytosis of obese subjects. Int J Obes Relat Metab Disord 24:1212–1216, 2000[Medline]
  9. Molina A, Vendrell J, Gutierrez C, Simon I, Masdevall C, Soler J, Gomez JM: Insulin resistance, leptin and TNF-alpha system in morbidly obese women after gastric bypass. Obes Surg 13:615–621, 2003[Medline]
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  11. Kooijman R, Coppens A, Hooghe-Peters E: IGF-I inhibits spontaneous apoptosis in human granulocytes. Endocrinology 143:1206–1212, 2002[Abstract/Free Full Text]
  12. De Marinis L, Bianchi A, Mancini A, Gentilella R, Perrelli M, Giampietro A, Porcelli T, Tilaro L, Fusco A, Valle D, Tacchino RM: Growth hormone secretion and leptin in morbid obesity before and after biliopancreatic diversion: relationships with insulin and body composition. J Clin Endocrinol Metab 89:174–180, 2004[Abstract/Free Full Text]
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