HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Online-Only Appendix All Versions of this Article: dc06-1458v1 30/6/1406    most recent Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ghanim, H. Articles by Dandona, P. Search for Related Content PubMed PubMed Citation Articles by Ghanim, H. Articles by Dandona, P. Social Bookmarking                What's this?

Orange Juice or Fructose Intake Does Not Induce Oxidative and Inflammatory Response

¹Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, 3 Gates Circle, Buffalo, NY 14209

pdandona{at}KaleidaHealth.org

ABSTRACT

Background: We have previously shown that 300 kcal from glucose intake induces a significant increase in reactive oxygen species (ROS) generation and nuclear factor -B (NFB) binding in the circulating mononuclear cells (MNC) in healthy normal subjects.

Hypothesis: We have now hypothesized that the intake of 300 Calories as (1.) orange juice or (2.) fructose, the other major carbohydrate in orange juice, would induce a significantly smaller response than that of glucose.

Investigation: Four groups (8 subjects each) of normal weight subjects were given a 300 Calories drink of glucose (75g), or fructose (75g) or orange juice or water sweetened with saccharin (control group) to drink and blood samples collected. There was a significant increase in ROS generation by MNC (by 130±18%, P< 0.001) and PMN (by 95±22%, P< 0.01) and in NFB binding in MNC by 82±16% (P<0.01) over the baseline after 2 hours of glucose intake. These changes were absent following fructose, orange juice or water intake. There was a significantly lower ROS generation and NFB binding following orange juice, fructose and water when compared with glucose (P<0.001 for all). Furthermore, incubation of MNC, in vitro, with 50mM of the flavonoids hesperetin or naringenin reduced ROS generation by 52±7% and 77±8 % (P<0.01), respectively, while fructose or ascorbic acid did not cause any change.

Conclusion: Caloric intake in the form of orange juice or fructose does not induce either oxidative or inflammatory stress, possibly due to its flavonoids content, and might, therefore, represent a potentially safe energy source.

CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?