Elevated Cholesteryl Ester Transfer Protein Activity in IDDM Men Who Smoke: Possible Factor for Unfavorable Lipoprotein Profile
- Robin P F Dullaart, MD,
- Johanna E M Groener, PhD,
- Bert D Dikkeschei, PhD,
- D Willem Erkelens, MD and
- Hieronymus Doorenbos, MD
- Departments of Endocrinology and Clinical Chemistry, University Hospital Croningen Department of Biochemistry I, Erasmus University Rotterdam Department of Internal Medicine, University Hospital Utrecht, Netherlands
- Address correspondence and reprint requests to Robin P.F. Dullaart, MD, Department of Endocrinology, University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Croningen, Netherlands.
Abstract
Objectives To determine the effect of cigarette smoking on the activity of cholesteryl ester transfer protein (CETP) and high-density (HDL), low-density (LDL), and very-low-density (VLDL) lipoproteins in insulindependent diabetic (IDDM) men with microvascular complications.
Research Design and Methods We perfomed a case-control study in a referral-based diabetes clinic on a sequential sample of 9 cigarettesmoking and 12 nonsmoking IDDM men with microvascular complications and 12 nonsmoking control men. CETP activity was determined in each serum with an isotope assay with exogenous cholesteryl esterlabeled LDL and HDL. The method is independent of the endogenous lipoprotein present in serum.
Results The HDL-cholesterol (VLDL and LDL) ratio was lower in the smoking diabetic men than in the other groups (P < 0.05 vs. the nonsmoking diabetic men and P <0.01 vs. the control subjects). CETP activity was 70% higher in the smoking diabetic men than in the control subjects (P <0.01) and 30% higher than in the nonsmoking diabetic men (P <0.05). The HDL-cholesterol (VLDL and LDL) ratio and the apolipoprotein A-l-B ratio were inversely correlated to CETP activity in the diabetic patients (r = −0.52, P <0.02 and r = −0.45, P <0.05, respectively).
Conclusions CETP activity is increased in cigarette-smoking IDDM men with microvascular complications. High CETP activity may contribute to the unfavorable lipoprotein profile in these patients.
- Received June 4, 1990.
- Accepted November 5, 1990.
- Copyright © 1991 by the American Diabetes Association
