MPO Activity and Generation of Active O2 Species in Leukocytes From Poorly Controlled Diabetic Patients
- Noriyuki Sato, MD,
- Hiroyuki Shimizu, MD,
- Kunihiko Suwa, MD,
- Yohnosuke Shimomura, MD,
- Isao Kobayashi, MD and
- Masatomo Mori, MD
- First Department of Internal Medicine and Department of Laboratory Medicine, Gunma University School of Medicine Maebashi, Japan
- Address Correspondence and reprint requests to Hiroyuki Shimizu, MD, PHD, First Department of Internal Medicine, Gunma University School of Medicine, 3-39-22 Showa-Machi Maebashi, 371 Japan.
Abstract
OBJECTIVE This study was undertaken to determine which part of ROI generation is reduced in the neutrophils from patients with NIDDM.
RESEARCH DESIGN AND METHODS Superoxide anion (O2−) production, LDCL activity in response to opsonized zymosan, and MPO activity were measured in leukocytes of poorly controlled NIDDM patients (FBG >8.89 mM, HbA1 >10%).
RESULTS In diabetic subjects, both O2− production and LDCL activity assessed with initial slope gradient and peak value were significantly reduced. MPO activity was also decreased in diabetic subjects, and there was a significant correlation between HbA1 levels and MPO activity of diabetic subjects.
CONCLUSIONS This study demonstrated that every step in leukocyte ROI generation should be reduced in the leukocytes from poorly controlled diabetic patients.
- Received August 1, 1991.
- Accepted December 22, 1991.
- Copyright © 1992 by the American Diabetes Association
