HLA-DQβl Typing and Non-Asp57 Alleles in the Aborigine Population of Senegal
- Maryline Chauffert, PHD,
- Aynina Cisse, PHD,
- Didier Chevenne, PHD,
- Béatrice Parfait, PHD,
- Simone Michel, BS and
- François Trivin, MD
- Laboratoire de Biochimie, Fondation Hôpital Saint-Joseph Paris, France
- Laboratoire, Centre de Traumatologie d'Orthopédie, et de Réadaptation Fonctionnelle, Dakar, Senegal
- Address correspondence and reprint requests to Maryline Chauffert, PhD, Laboratoire de Biochimie, Fondation Hôpital Saint-Joseph, 185 rue Raymond Losserand, 75674 Paris Cedex 14, France.
Abstract
OBJECTIVE To investigate, in Senegalese subjects, the frequency of human leukocyte antigen (HLA)-DQβ1 alleles and their role in susceptibility to insulin-dependent diabetes mellitus (IDDM).
RESEARCH DESIGN AND METHODS HLA-DQβ1 typing was done in 55 IDDM subjects and 118 nondiabetic control subjects by means of polymerase chain reaction restriction fragment length polymorphism.
RESULTS All;eles bearing a codon for an Asp residue at position 57 in the DQ β-chain were associated with a significantly lower risk of IDDM. Alleles 0201 and 0302 (Ala57) were positively associated with diabetes, but allele 0501 (Val57) was less frequent in IDDM subjects than in control subjects.
CONCLUSIONS HLA-DQβl alleles may be genetic susceptibility markers for IDDM in the Senegalese population, as they are in Caucasian populations.
- Received July 29, 1994.
- Accepted January 11, 1995.
- Copyright © 1995 by the American Diabetes Association
