Variants of the Fatty Acid-Binding Protein 2 Gene Are Not Associated With Coronary Heart Disease in Nondiabetic Subjects and in Patients With NIDDM
- Laura Saarinen,
- Arto Pulkkinen, MD,
- Anu Kareinen, MD,
- Sami Heikkinen, MSC,
- Seppo Lehto, MD and
- Markku Laakso, MD
- Address correspondence and reprint requests to Markku Laakso, MD, Department of Medicine, University of Kuopio, 70210 Kuopio, Finland. E-mail: markku.laakso{at}uku.fi.
Abstract
OBJECTIVE To investigate the association of variants of the fatty acid-binding protein (FABP) 2 gene with coronary heart disease (CHD) in nondiabetic subjects and in patients with NIDDM.
RESEARCH DESIGN AND METHODS Cross-sectional study included 135 nondiabetic and 79 NIDDM subjects with stenosis (>50%) in at least two coronary arteries. A group of 81 healthy nondiabetic men without CHD served as a control population. All exons and intron-exon junctions of the FABP2 gene were amplified with the polymerase chain reaction, and variants were screened with the single-strand conformation polymorphism analysis.
RESULTS The allele frequency of an amino acid polymorphism (alanine→threonine) in codon 54 of exon 2 of the FABP2 gene was 0.26 in nondiabetic subjects with CHD and 0.27 in NIDDM subjects with CHD. Other variants (GTA 118 GTC, GCGCA→GCACA in the 3'- noncoding region, and the number of ATT repeats in intron 2) also did not associate with CHD.
CONCLUSIONS The variants of the FABP2 gene are not likely to contribute to the risk of CHD in Finnish nondiabetic and NIDDM subjects.
- Received October 13, 1997.
- Revision received January 26, 1998.
- Accepted January 26, 1998.
- Copyright © 1998 by the American Diabetes Association
