Relation Between Serum 3-Deoxyglucosone and Development of Diabetic Microangiopathy
- Hitomi Kusunoki, MD1,
- Satoshi Miyata, MD, PHD1,
- Takeshi Ohara, MD, PHD12,
- Bing-Fen Liu, MD, PHD1,
- Atsuko Uriuhara, MD1,
- Hiroshi Kojima, MD1,
- Kotaro Suzuki, MD1,
- Hiroyuki Miyazaki, MD1,
- Yumiko Yamashita, MD1,
- Kayo Inaba, MD1 and
- Masato Kasuga, MD, PHD1
- 1Division of Diabetes, Digestive and Kidney diseases, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan
- 2Department of Internal Medicine, National Hyogo Chuo Hospital, Sanda, Japan
Abstract
OBJECTIVE—3-Deoxyglucosone (3-DG), a highly reactive intermediate of the glycation reaction, has been suggested to contribute to the development of diabetes complications. To verify this hypothesis, we assessed the relation between serum 3-DG concentrations and the severity of diabetic microangiopathy in diabetic patients.
RESEARCH DESIGN AND METHODS—We conducted a high-performance liquid chromatography assay to determine the serum 3-DG concentrations of 110 diabetic patients with different degrees of severity of diabetic microangiopathy and 57 age-matched control subjects.
RESULTS—The fasting serum 3-DG level in diabetic patients was significantly (P < 0.001) higher than that in control subjects (353 ± 110 vs. 199 ± 53 nmol/l). The 3-DG levels were significantly (P < 0.001) elevated even in the diabetic patients showing normoalbuminuria (n = 62, 322 ± 79 nmol/l) compared with control subjects. The 3-DG levels were further elevated in the patients with microalbuminuria (n = 30, 383 ± 146 nmol/l) and overt proteinuria (n = 18, 410 ± 100 nmol/l) (P = 0.027 and P < 0.001 vs. normoalbuminuria group, respectively). This phenomenon was basically reproduced in a category of retinopathy. Furthermore, the diabetic patients with low nerve conduction velocity showed a tendency to display higher 3-DG levels.
CONCLUSIONS—The present results show that the fasting serum 3-DG level is elevated in diabetic patients and that the patients with relatively higher 3-DG levels were prone to suffer from more severe complications, indicating a possible association of 3-DG with diabetic microangiopathy.
- 3-DG, 3-deoxyglucosone
- AGE, advanced glycation end product
- FCV, F-wave conduction velocity
- HPLC, high-performance liquid chromatography
- MCV, motor nerve conduction velocity
- SCV, sensory nerve conduction velocity
Footnotes
-
Address correspondence and reprint requests to Satoshi Miyata, MD, Division of Diabetes, Digestive and Kidney Diseases, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. E-mail: miyata{at}med.kobe-u.ac.jp.
Received for publication 15 June 2002 and accepted in revised form 3 March 2003.
A table elsewhere in this issue shows conventional and Système International (SI) units and conversion factors for many substances.
- DIABETES CARE
