The Xylosyltransferase I Gene Polymorphism c.343G>T (p.A125S) Is a Risk Factor for Diabetic Nephropathy in Type 1 Diabetes
- Sylvia Schön, PHD1,
- Christian Prante, MS1,
- Claudia Bahr, MS1,
- Lise Tarnow, MD, DMSC2,
- Joachim Kuhn, PHD1,
- Knut Kleesiek, MD1 and
- Christian Götting, PHD1
- 1Institut für Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Bad Oeynhausen, Germany
- 2Steno Diabetes Center, Gentofte, Denmark
- Address correspondence and reprint requests to Christian Götting, Institut für Laboratoriums und Transfusionsmedizin, Herz und Diabeteszentrum Nordrhein-Westfalen, Georgstraβe 11, 32545 Bad Oeynhausen, Germany. E-mail: cgoetting{at}hdz-nrw.de
Abstract
OBJECTIVE—Xylosyltransferase I (XT-I) is the chain-initiating enzyme in the biosynthesis of proteoglycans in basement membranes. It catalyzes the transfer of xylose to selected serine residues in the core protein. The XYLT-II gene codes for a protein highly homologous to XT-I. Proteoglycans are important components of basement membranes and are responsible for their permeability properties. Type 1 diabetic patients have an altered proteoglycan metabolism, which results in microvascular complications. Thus, genetic variations in the xylosyltransferase genes might be implicated in the initiation and progression of these complications.
RESEARCH DESIGN AND METHODS—Genotyping of four genetic variations in the genes XYLT-I and XYLT-II was performed in 912 type 1 diabetic patients (453 with and 459 without diabetic nephropathy) using restriction fragment–length polymorphism.
RESULTS—The distribution of the c.343G>T polymorphism in XYLT-I is significantly different between patients with and without diabetic nephropathy (P = 0.03). T-alleles were more frequent in patients with diabetic nephropathy (odds ratio 2.47 [95% CI 1.04–5.83]). The allelic frequencies of the other investigated XYLT-I and XYLT-II variations (XYLT-I: c.1989T>C in exon 9; XYLT-II: IVS6–9T>C and IVS6–14_IVS6–13insG in intron 5; and c.2402C>G: p.T801R in exon 11) were not different between patients with and without diabetic nephropathy.
CONCLUSIONS—The XYLT-I c.343G>T polymorphism contributes to the genetic susceptibility to development of diabetic nephropathy in type 1 diabetic patients.
Footnotes
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- Accepted July 13, 2006.
- Received February 10, 2006.
- DIABETES CARE
