Preβ1-HDL Concentration Is a Predictor of Carotid Atherosclerosis in Type 2 Diabetic Patients
- Satoshi Hirayama, MD1,
- Takashi Miida, MD2,
- Osamu Miyazaki, PHD3 and
- Yoshifusa Aizawa, MD1
- 1Division of Endocrinology and Metabolism, Niigata University, Niigata, Japan
- 2Division of Clinical Preventive Medicine, Niigata University, Niigata, Japan
- 3Daiichi Pure Chemicals, Ibaraki, Japan
- Address correspondence and reprint requests to Takashi Miida, Division of Clinical Preventive Medicine, Niigata University, Asahimachi 1-757, Niigata, 951-8510, Japan. E-mail: miida{at}med.niigata-u.ac.jp
- CAD, cardiovascular disease
- LCAT, lecithin-cholesterol acyltransferase
- max IMT, greatest intima-media thickness
Pre-β1-HDL is a minor HDL subfraction that stimulates cholesterol efflux from cell membranes (1,2). However, the fasting preβ1-HDL concentration is elevated in patients with coronary artery disease (CAD), hyperlipidemia, and obesity and in hemodialysis patients (3–7). We examined whether the preβ1-HDL concentration is elevated in type 2 diabetic patients and whether elevated preβ1-HDL concentration is a predictor of carotid atherosclerosis.
RESEARCH DESIGN AND METHODS—
We measured the preβ1-HDL concentration in 30 patients with type 2 diabetes (mean ± SD age 58.5 ± 12.3 years, 13 men and 17 women) and in 30 age- and sex-matched healthy control subjects. We excluded patients receiving hypolipidemic agents and those with renal dysfunction. The diabetic group had a long duration of disease (9.9 ± 8.6 years), high A1C levels (10.4 ± 2.0%), and high BMI (26.1 ± 6.5 kg/m2). The preβ1-HDL in frozen plasma, pretreated with sucrose solution for stabilization, was measured using an immunoassay (8). In the diabetic group, we evaluated the severity of carotid atherosclerosis using ultrasonography with a 7.5-MHz probe. The greatest intima-media thickness (max IMT) and plaque score were determined as previously reported (9).
RESULTS—
The diabetic group had higher triglyceride concentrations than the control subjects (166.9 ± 138.4 vs. 90.9 ± 28.6 mg/dl, P < 0.01), while the two groups did not differ significantly in total cholesterol (215.1 ± 49.9 vs. 201.0 ± 20.9 mg/dl), LDL cholesterol (135.9 ± 37.1 vs. 125.3 ± 17.9 mg/dl), or HDL cholesterol (55.5 ± …
