Orange Juice or Fructose Intake Does Not Induce Oxidative and Inflammatory Response
- Husam Ghanim, PHD,
- Priya Mohanty, MD,
- Ram Pathak, MD,
- Ajay Chaudhuri, MD,
- Chang Ling Sia, BSC and
- Paresh Dandona, MD, PHD
- From the Division of Endocrinology, Diabetes and Metabolism, State University of New York at Buffalo and Kaleida Health, Buffalo, New York
- Address correspondence and reprint requests to Paresh Dandona, BSc, MB, BS, DPhil, FRCP, Director, Diabetes-Endocrinology Center of Western NY, Chief of Endocrinology, State University of New York at Buffalo, 3 Gates Circle, Buffalo, NY 14209. E-mail: pdandona{at}kaleidahealth.org
Abstract
OBJECTIVE—We have previously shown that 300 kcal from glucose intake induces a significant increase in reactive oxygen species (ROS) generation and nuclear factor-κB (NF-κB) binding in the circulating mononuclear cells in healthy normal subjects. We hypothesized that the intake of 300 calories as orange juice or fructose, the other major carbohydrate in orange juice, would induce a significantly smaller response than that of glucose.
RESEARCH DESIGN AND METHODS— Four groups (eight subjects each) of normal-weight subjects were given a 300-cal drink of glucose (75 g), fructose (75 g), or orange juice or water sweetened with saccharin (control group) to drink, and then blood samples were collected.
RESULTS—There was a significant increase in ROS generation by mononuclear cells (by 130 ± 18%, P < 0.001), polymorph nuclear cells (by 95 ± 22%, P < 0.01), and in NF-κB binding in mononuclear cells by 82 ± 16% (P < 0.01) over the baseline after 2 h of glucose intake. These changes were absent following fructose, orange juice, or water intake. There was significantly lower ROS generation and NF-κB binding following orange juice, fructose, and water compared with glucose (P < 0.001 for all). Furthermore, incubation of mononuclear cells in vitro with 50 mmol/l of the flavonoids hesperetin or naringenin reduced ROS generation by 52 ± 7% and 77 ± 8% (P < 0.01), respectively, while fructose or ascorbic acid did not cause any change.
CONCLUSIONS—Caloric intake in the form of orange juice or fructose does not induce either oxidative or inflammatory stress, possibly due to its flavonoids content and might, therefore, represent a potentially safe energy source.
- CRP, C-reactive protein
- IKK, inhibitor κB kinase
- NF-κB, nuclear factor-κB
- ROS, reactive oxygen species
Footnotes
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Published ahead of print at http://care.diabetesjournals.org on 23 March 2007. DOI: 10.2337/dc06-1458.
Additional information for this article can be found in an online appendix at http://dx.doi.org/10.2337/dc06-1458.
A table elsewhere in this issue shows conventional and Système International (SI) units and conversion factors for many substances.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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- Accepted March 10, 2007.
- Received July 11, 2006.
- DIABETES CARE
