Increased Shear Rate Resistance and Fastest Kinetics of Erythrocyte Aggregation in Diabetes Measured With Ultrasound

  1. Guy Cloutier, PHD12,
  2. Audrey Zimmer, MSC1,
  3. François T.H. Yu, MSC1 and
  4. Jean-Louis Chiasson, MD3
  1. 1Laboratory of Biorheology and Medical Ultrasonics, Research Center, University of Montreal Hospital, Montreal, Quebec, Canada
  2. 2Department of Radiology, Radio-Oncology and Nuclear Medicine and the Institute of Biomedical Engineering, University of Montreal, Quebec, Canada
  3. 3Research Center, University of Montreal Hospital, Montreal, Quebec, Canada, and the Department of Medicine, University of Montreal, Montreal, Quebec, Canada
  1. Corresponding author: Dr. Guy Cloutier, guy.cloutier{at}umontreal.ca

Abstract

OBJECTIVE—To measure with ultrasound the increased erythrocyte aggregation (EA) kinetics and adhesion energy between erythrocytes in patients with type 2 diabetes and poor metabolic control.

RESEARCH DESIGN AND METHODS—Blood samples were analyzed in a Couette rheometer at 32 MHz following shear rate reductions from 500 s−1 to residual shears of 0 (stasis), 1, 2, 10, 50, 100, and 200 s−1. The increase in EA was determined with the integrated backscatter coefficient as a function of time and shear rate.

RESULTS—The time required to form aggregates was shorter in diabetic patients at shear rates below 200 s−1 (P < 0.01). Erythrocytes formed larger aggregates in diabetic patients than in control subjects (P < 0.05 at 2 to 100 s−1).

CONCLUSIONS—Ultrasound can potentially noninvasively demonstrate, in vivo and in situ, the impact of local abnormal EA on arteriovenous flow disorders in diabetes.

Footnotes

  • Published ahead of print at http://care.diabetesjournals.org on 28 March 2008.

    Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

    The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

    • Accepted March 26, 2008.
    • Received September 12, 2007.
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  1. Published online before print March 28, 2008, doi: 10.2337/dc07-1802 Diabetes Care July 2008 vol. 31 no. 7 1400-1402
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