Novel Soy Germ Pasta Improves Endothelial Function, Blood Pressure, and Oxidative Stress in Patients With Type 2 Diabetes
- Carlo Clerici, MD1,
- Elisabetta Nardi, MD1,
- Pier Maria Battezzati, MD2,
- Stefania Asciutti, MD1,
- Danilo Castellani, MD1,
- Nadia Corazzi, MD1,
- Vittorio Giuliano, MD1,
- Stefania Gizzi, MD1,
- Gabriele Perriello, MD3,
- Giorgio Di Matteo, MD3,
- Francesco Galli, MD4 and
- Kenneth D.R. Setchell, PHD5⇓
- 1Clinic of Gastroenterology and Hepatology, University of Perugia, Perugia, Italy
- 2Division of Internal Medicine VI, Department of Medicine, Surgery, and Dentistry, San Paolo School of Medicine, University of Milan, Milan, Italy
- 3Department of Internal Medicine and Endocrine and Metabolic Sciences (DIMISEM), University of Perugia, Perugia, Italy
- 4Institute of Biochemistry, University of Perugia, Perugia, Italy
- 5Department of Pathology and Laboratory Medicine, Cincinnati Children’s Hospital Medical Center and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio
- Corresponding author: Kenneth D.R. Setchell, .
OBJECTIVE To compare the effects of a novel soy germ–enriched pasta, containing isoflavone aglycons, with conventional pasta on endothelial function and cardiovascular risk markers in patients with type 2 diabetes (T2D).
RESEARCH DESIGN AND METHODS This randomized controlled double-blind crossover study compared one serving/day of soy germ pasta and conventional pasta for 8 weeks for effects on brachial artery flow-mediated vasodilation, blood pressure, plasma lipids, oxidized LDL cholesterol, 8-iso-PGF2α, total antioxidant capacity (TAC), glutathione (GSH), and homocysteine.
RESULTS Isoflavone-enriched pasta significantly improved arterial stiffness (P = 0.005) and reduced systolic (P = 0.026) and diastolic (P = 0.017) blood pressures. Plasma TAC increased (P = 0.0002), oxidized LDL cholesterol decreased (P = 0.009), 8-iso-PGF2α decreased (P = 0.001), GSH levels increased (P = 0.0003), and homocysteine decreased (P = 0.009) consistent with a reduction in oxidative stress. No significant changes were observed with conventional pasta.
CONCLUSIONS Pasta enriched with biologically active isoflavone aglycons improved endothelial function and had beneficial effects on cardiovascular risk markers in patients with T2D.
The risk of cardiovascular disease and stroke is 3–4 times greater in patients with type 2 diabetes (T2D) than in the general population (1). It results from loss of normal endothelial function and is associated with increased oxidative stress, production of reactive oxygen species (2), and a decrease in antioxidative defense systems (3). Hypertension, dyslipidemia, and hyperhomocysteinemia also contribute to overall morbidity and mortality. In a recent study of patients with hypercholesterolemia, a soy germ–enriched pasta containing isoflavones, in mainly the biologically active aglycon form, significantly improved serum lipids and other markers of cardiovascular risk, including arterial reactivity, beyond that of an American Heart Association Step II diet alone (4). Our objective was to determine whether similar effects from this novel food could be attained in patients with T2D.
RESEARCH DESIGN AND METHODS
This 20-week study enrolled 26 adults with T2D (13 men/13 women, aged 62 ± 7 years) in a randomized double-blind placebo-controlled crossover design. None had major complications of diabetes and all were on an American Diabetes Association diet. Prior use of estrogen therapy, oral corticosteroids, antioxidant supplements, soy foods or isoflavone supplements, a BMI >35, and smoking were exclusion criteria. The study was conducted with informed consent according to ethical guidelines for research on humans and was approved by the human ethics committee of the University of Perugia School of Medicine.
Two types of dried pasta were used: a soy germ–enriched pasta (Aliveris s.r.l., Perugia, Italy) containing 31–33 mg of total isoflavones per serving predominantly in aglycon form (Pasta+) and conventional pasta (Pasta−), with both packaged identically (4).
Patients were randomized to two groups. One group consumed one serving/day of soy germ–enriched pasta (80 g dry pasta) for 8 weeks followed by conventional pasta for 8 weeks, with a 4-week washout between. The other group consumed these pasta types in reverse sequence. Overnight fasting blood samples were collected at baseline, after washout, and at the end of the diet periods for serum/plasma biochemistries measured by standard methods. Blood pressure was measured and endothelial function assessed from changes in brachial artery flow-mediated vasodilation using ultrasound imaging of artery diameter during reactive hyperemia (5). Methods for data analysis are detailed in the Supplementary Data.
Of the 26 patients enrolled, 6 were withdrawn (4 whose drug therapies were altered, 1 who took antioxidants, and 1 who was noncompliant to the diets). The mean BMI of the remaining subjects (10 women, 10 men) was 28.3 ± 3.5 kg/m2, fasting glucose was 122 ± 33 mg/dL, and HbA1c was 6.8 ± 0.65%. After randomization, there were no significant differences between the groups (Pasta+/Pasta− vs. Pasta−/Pasta+) in these measures.
Plasma isoflavones were undetectable at baseline and when consuming Pasta−. When consuming Pasta+, serum daidzein, glycitein, and genistein concentrations ranged 12–255 nmol/L, 11–128 nmol/L, and 15–167 nmol/L, respectively, consistent with previously reported data (4).
Brachial artery flow-mediated vasodilation increased ∼5% after consuming Pasta+ and decreased with Pasta− (Fig. 1 and Supplementary Data); this difference was highly significant (P = 0.0005). Consistent with improved endothelial function was a significant reduction in systolic (P = 0.026) and diastolic (P = 0.017) blood pressures. Pasta+ lowered systolic blood pressure from 133 ± 1 at baseline to 126 ± 12 mmHg, and diastolic blood pressure from 79 ± 9 at baseline to 73 ± 10 mmHg. Pasta− had no effect on blood pressure.
The effect of the two pastas on serum total cholesterol was in the opposite direction, with the difference being significant (P = 0.025). Favorable, albeit not significant, differential trends were observed with Pasta+ on serum LDL cholesterol, HDL cholesterol, and triglyceride levels.
Several markers of oxidative stress improved after consuming Pasta+ (Fig. 1 and Supplementary Data). Plasma total antioxidant capacity (TAC) increased significantly (P = 0.0002) in comparison with Pasta−. Plasma 8-iso-PGF2α concentration was reduced (P = 0.001) and glutathione (GSH) increased (P = 0.0003). Consistent with these changes was a reduction in lipid peroxidation, as observed by a decrease in plasma oxidized LDL cholesterol (P = 0.009). Serum homocysteine concentration was lower (P = 0.017) after Pasta+ compared with Pasta−, but there was no effect of either pasta on serum cysteine or interleukin-6 levels. At baseline, all patients were in good glycemic control and, consequently, neither pasta influenced plasma insulin, glucose, or HbA1c levels.
This novel soy germ pasta was developed with the objective of delivering biologically active isoflavone aglycons (4) at levels typically found in soy foods in the Asian diet (6) and not to satisfy the 1999-approved U.S. Food and Drug Administration cardiovascular health claim for soy protein (7). A high dietary intake of isoflavone aglycons may explain why soy foods appear beneficial to Asians (8) and why pure aglycon supplements, such as genistein (9) or S-(-)equol (10), are proving efficacious. Because Italians consume pasta almost daily, this facilitated dietary compliance, which was confirmed by the expected increase in plasma isoflavone concentrations (4) or from returned package counts.
Although no significant changes were observed with either pasta on serum total and LDL cholesterol, which were relatively normal at baseline, serum triglycerides showed a decreasing trend that was greater with Pasta+ than Pasta−. Despite a lack of effect on serum lipids, impressive effects on other important surrogate markers of cardiovascular risk were observed. Endothelial dysfunction, an early marker of cardiovascular disease, has prognostic value in assessing cardiovascular risk (11) and was significantly improved by Pasta+. This finding corroborates previous data in hypercholesterolemic patients (4) and is consistent with the vasodilatory effects of isoflavone aglycons (9). With Pasta+, highly significant 8 and 7% reductions, respectively, in systolic and diastolic blood pressures occurred, and considering the small sample-size, the magnitude of the effect on endothelial function and blood pressure is impressive and clinically relevant.
Because isoflavones have antioxidant activity (12), the finding of highly significant beneficial effects of Pasta+ on several markers of oxidative stress, and on serum GSH, a key constituent of the antioxidant defense system, was not unexpected but important given that increased oxidative stress is seen in T2D (13). Furthermore, hyperhomocysteinemia is an independent risk factor for cardiovascular disease in diabetes (14), and Pasta+ lowered serum levels of this atherogenic and prothrombotic amino acid.
In summary, this isoflavone-enriched soy germ pasta, unlike conventional pasta, significantly improved multiple markers of cardiovascular risk in patients with T2D. Dietary inclusion of this novel pasta may provide benefits to diabetic patients beyond those achieved by an American Diabetes Association diet alone.
The authors acknowledge the support of the Aliveris s.r.l. Company (Perugia, Italy) and the Bianconi Company (Bastardo, Umbria, Italy) for providing the soy germ pasta and conventional pasta used in this study. C.C. and K.D.R.S. disclose financial interests in Aliveris s.r.l. No other potential conflicts of interest relevant to this article were reported.
C.C. and K.D.R.S. were principal investigators responsible for the conception and design of the study and prepared the manuscript with support from the coauthors. E.N. was responsible for recruitment, enrollment, clinical care, and follow-up of patients and performed the clinical and biochemical tests, data collection, and processing. P.M.B. performed all the statistical analysis of data. S.A., D.C., and F.G. performed the clinical and biochemical tests, data collection, and processing. N.C. was responsible for recruitment, enrollment, clinical care, and follow-up of patients. V.G. and S.G. performed the clinical and biochemical tests, data collection, and processing. G.P. and G.D.M. were responsible for recruitment, enrollment, clinical care, and follow-up of patients.
This article contains Supplementary Data online at http://care.diabetesjournals.org/lookup/suppl/doi:10.2337/dc11-0495/-/DC1.
- Received March 16, 2011.
- Accepted May 27, 2011.
- © 2011 by the American Diabetes Association.
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