Lactation Intensity and Postpartum Maternal Glucose Tolerance and Insulin Resistance in Women With Recent GDM
The SWIFT cohort
- Erica P. Gunderson, PHD1⇓,
- Monique M. Hedderson, PHD1,
- Vicky Chiang, MS1,
- Yvonne Crites, MD2,
- David Walton, MD2,
- Robert A. Azevedo, MD2,
- Gary Fox, MD2,
- Cathie Elmasian, MD2,
- Stephen Young, MD2,
- Nora Salvador, MD2,
- Michael Lum, MD2,
- Charles P. Quesenberry, PHD1,
- Joan C. Lo, MD1,
- Barbara Sternfeld, PHD1,
- Assiamira Ferrara, MD, PHD1 and
- Joseph V. Selby, MD1
- 1Division of Research, Kaiser Permanente Northern California, Oakland, California
- 2Kaiser Permanente Northern California Medical Group, Oakland, California
- Corresponding author: Erica P. Gunderson, Erica.Gunderson@kp.org.
OBJECTIVE To examine the association between breastfeeding intensity in relation to maternal blood glucose and insulin and glucose intolerance based on the postpartum 2-h 75-g oral glucose tolerance test (OGTT) results at 6–9 weeks after a pregnancy with gestational diabetes mellitus (GDM).
RESEARCH DESIGN AND METHODS We selected 522 participants enrolled into the Study of Women, Infant Feeding, and Type 2 Diabetes (SWIFT), a prospective observational cohort study of Kaiser Permanente Northern California members diagnosed with GDM using the 3-h 100-g OGTT by the Carpenter and Coustan criteria. Women were classified as normal, prediabetes, or diabetes according to American Diabetes Association criteria based on the postpartum 2-h 75-g OGTT results.
RESULTS Compared with exclusive or mostly formula feeding (>17 oz formula per 24 h), exclusive breastfeeding and mostly breastfeeding (≤6 oz formula per 24 h) groups, respectively, had lower adjusted mean (95% CI) group differences in fasting plasma glucose (mg/dL) of −4.3 (−7.4 to −1.3) and −5.0 (−8.5 to −1.4), in fasting insulin (μU/mL) of −6.3 (−10.1 to −2.4) and −7.5 (−11.9 to −3.0), and in 2-h insulin of −21.4 (−41.0 to −1.7) and −36.5 (−59.3 to −13.7) (all P < 0.05). Exclusive or mostly breastfeeding groups had lower prevalence of diabetes or prediabetes (P = 0.02).
CONCLUSIONS Higher intensity of lactation was associated with improved fasting glucose and lower insulin levels at 6–9 weeks’ postpartum. Lactation may have favorable effects on glucose metabolism and insulin sensitivity that may reduce diabetes risk after GDM pregnancy.
Lactogenesis has favorable effects on maternal cardiometabolic blood profiles, including a less atherogenic lipid profile (1) and lower blood glucose and insulin concentrations (2,3), as a result of the noninsulin-mediated cellular uptake of glucose for milk production. Some evidence suggests that lactation may be associated with greater insulin sensitivity (2). Among 809 Latinas with recent gestational diabetes mellitus (GDM), the lactating group had lower mean fasting and 2-h postglucose and higher HDL cholesterol at 4–12 weeks’ postpartum compared with the nonlactating group (4). A second study reports improved pancreatic β-cell function among 14 lactating versus 12 nonlactating women with previous GDM assessed via the disposition index (insulin sensitivity multiplied by acute insulin response to glucose) (5).
Lactation intensity (e.g., degree of milk feed supplementation), to our knowledge, has never been examined in relation to maternal postpartum glucose tolerance, metabolic profile, or insulin resistance among women with a history of GDM. In addition, the few studies comparing metabolic parameters among lactating versus nonlactating postpartum women with recent GDM are limited to Latinas (4) or fewer than 30 non-Hispanic white women (5); there are currently no published data from racially/ethnically diverse cohorts. The objective of this analysis is to examine the association between intensity of breastfeeding and formula feeding in relation to blood glucose and insulin levels as well as glucose tolerance based on the postpartum 2-h 75-g oral glucose tolerance test (OGTT) among women with recent GDM who enrolled in the Study of Women, Infant Feeding, and Type 2 Diabetes (SWIFT), a Kaiser Permanente Northern California postpartum GDM cohort.
RESEARCH DESIGN AND METHODS
The analysis includes participants enrolled between September 2008 and March 2011 into SWIFT, an ongoing prospective observational cohort study of Kaiser Permanente Northern California members, who met American Diabetes Association criteria for the 3-h 100-g OGTT at 24–32 weeks’ gestation for a diagnosis of GDM and delivered a singleton, live birth ≥35 weeks’ gestation (6). Eligible participants had no known major medical conditions, provided information on duration and intensity of breastfeeding and formula feeding, and were free of diabetes at 6–9 weeks’ postpartum (confirmed by the 2-h 75-g OGTT) for inclusion in the follow-up cohort screened annually for diabetes. This prospective study enrolled women into one of two infant feeding groups: exclusive or mostly breastfeeding (giving ≤6 oz formula per 24 h) and exclusive or mostly formula feeding (giving ≥14 oz formula per 24 h), based on the infant feeding practices assessed via telephone using the women’s record of formula supplementation (amount and number of feedings per 24 h) from delivery through 4–6 weeks’ postpartum. At the 6–9 week postpartum enrollment visit (baseline), research staff queried women about their frequency of breastfeeding and formula supplementation (including quantity per 24 h) during the previous 7 days. The analytic sample consists of 522 women—505 free of diabetes and 17 classified with diabetes at 6–9 weeks’ postpartum based on the 2-h 75-g OGTT results.
Women provided written, informed consent prior to enrollment at the in-person examination at 6–9 weeks’ postpartum to obtain blood specimens, questionnaire data, and anthropometric measurements. In preparation for the 2-h 75-g OGTT, women were advised to consume adequate carbohydrates for the 3 days before the test, fast for at least 10 h before the test, and express their breast milk a few days before the test so they would have breast milk to feed their infant during the OGTT. Women reported the frequency and duration of breastfeeding during the fasting period before the OGTT, and those who breastfed their infant during the 2-h OGTT also reported the duration and number of breastfeeding episodes during the OGTT to the research assistant.
Kaiser Permanente Northern California electronic databases were used to retrieve prenatal laboratory results for GDM diagnosis, date of delivery, and length of gestation. Measurements of weight and height were performed by trained research assistants using standardized methods and equipment. Interviewer and self-administered questionnaires collected information on sociodemographics, hours fasting before the OGTT, medical conditions, contraception, and lifestyle behaviors, including infant feeding methods. On the basis of infant feeding data collected from delivery to enrollment (6–9 weeks’ postpartum), participants were classified into one of four infant feeding groups: 1) exclusive breastfeeding (no formula or other feeds); 2) mostly breastfeeding, defined as ≤6 oz of formula per 24 h; 3) mixed or inconsistent feeding of breast milk and formula, defined as 7–17 oz per 24 h or change in feeding status to increase formula; and 4) exclusive or mostly formula feeding, defined as >17 oz formula per 24 h. These categories were based on the average quantity of infant formula fed per 24 h from birth to 6 weeks (feeding diary) and the average amount of formula fed within 1 week before enrollment. We defined an intake of ≤6 oz of formula per day as mostly breastfeeding and >17 oz of formula per day as mostly formula feeding. These criteria are based on an average formula intake of 24 oz per day among infants aged 6–9 weeks (e.g., 450 kcal per day) (7,8). More than 17 oz of formula per day is estimated to be at least 70% of the overall intake, and ≤6 oz of formula per day is estimated to be ≤25% of the overall intake on average as formula.
Plasma glucose assays were performed enzymatically by the University of Washington Northwest Lipid Metabolism and Diabetes Research Laboratory (Seattle, WA) with the Hitachi 917 Autoanalyzer using the combined catalytic activities of hexokinase and glucose-6-phosphate-dehydrogenase. The assay of total immunoreactive insulin, or total insulin, is performed by a double-antibody radioimmunoassay developed in the Diabetes Endocrinology Research Center Immunoassay Core Laboratory (Seattle, WA). The assay is a 48-h polyethylene glycol–accelerated assay involving a primary antibody, guinea pig anti-human insulin, and a secondary antibody, goat anti–guinea pig immunoglobulin. The guinea pig anti-human insulin antibody is available in a very large quantity as produced from the laboratory, therefore ensuring consistency of the assay throughout the years. Assay precision is excellent, with a coefficient of variation of 4.5% for the high quality control and 6.9% for the low quality control.
Glucose tolerance classification
Women were classified by glucose tolerance as follows: normal; glucose intolerant (prediabetes defined as impaired fasting glucose between 100 and 125 mg/dL and/or impaired glucose tolerance for 2-h 75-g postglucose between 140 and 199 mg/dL); or diabetes based on the American Diabetes Association diagnostic criteria for the 75-g OGTT (fasting ≥126 mg/dL and/or 2 h ≥200 mg/dL), which included a repeat test on a separate occasion for women with elevated values (9).
Insulin sensitivity and resistance indices
We calculated indices of insulin resistance (homeostasis model assessment of insulin resistance [HOMA-IR]) and insulin sensitivity (insulin sensitivity index [ISI 0, 120]) using data obtained from 0 and 120 min during the OGTT at 6–9 weeks’ postpartum (10,11): HOMA-IR = (G0 × I0)/22.5 and ISI 0, 120 = (m/MPG)/log MSI, where G0 = fasting glucose, I0 = fasting insulin, G120 = glucose post 2-h OGTT, I120 = insulin post 2-h OGTT,MPG = (G0 + G120)/2, and MSI = (I0 + I120)/2, where MPG is mean of fasting and 2-h glucose concentrations (mg/dL) and MSI is mean of fasting and 2-h insulin concentrations (mU/L).
Differences in participant characteristics by infant feeding groups were assessed using χ2 statistics for categorical variables (race, education, BMI, and breastfeeding during OGTT or fasting period) and by comparison of means for continuous variables (blood glucose, insulin, HOMA-IR, and ISI 0, 120) using F statistics from ANOVA. All P values presented are for two-sided tests; statistical significance was defined as P < 0.05.
Unadjusted and multivariable adjusted means (95% CI) and adjusted mean group differences in measures of glucose tolerance among the four infant feeding groups were estimated from linear regression models (ANOVA, F statistics) adjusted for covariates. Statistically significant P values and CIs were corrected for multiple comparisons of infant feeding groups using the Dunnett procedure in SAS for Windows 9.1.3 (SAS Institute Inc., Cary, NC). Covariates evaluated as potential confounders based on a priori hypotheses included race/ethnicity, maternal BMI, education, parity, and age. We also examined time fasting and breastfeeding during the fasting period as potential confounders based on 10% change in model coefficients for infant feeding groups.
Participants’ age ranged from 21 to 45 years with a mean (SD) age of 33.2 (5.0) years, and overall race/ethnicity of the cohort was 73% minority (36% Asian, 8% black, and 29% Hispanic). Among the analytic sample of 522 women (Table 1), 211 reported exclusively breastfeeding, 99 reported mostly breastfeeding (≤6 oz formula per 24 h), 77 reported mixed breast milk and formula (7–17 oz per 24 h) or inconsistent feeding, and 135 reported exclusive or mostly formula feeding (>17 oz per 24 h). Women who exclusively or mostly breastfed their infants had higher education attainment and were more likely to breastfeed during the OGTT, have lower BMI at 6–9 weeks’ postpartum, and be of non-Hispanic white race. The amount of formula provided to the infant within a 24-h period was higher among formula feeding than breastfeeding groups as expected (P < 0.001). The time interval spent breastfeeding during the fasting period and during the OGTT was higher among exclusively and mostly breastfeeding groups than other groups.
Plasma glucose and insulin and insulin resistance or sensitivity indices (HOMA-IR and ISI 0, 120) from unadjusted and adjusted multivariable linear regression models followed a dose-response pattern with increasing means directly associated with higher quantities of formula feeding (Table 2). Unadjusted and fully adjusted means (95% CI) for fasting plasma glucose, fasting plasma insulin, 2-h insulin concentrations, and HOMA-IR levels were significantly lower among exclusive breastfeeding and mostly breastfeeding groups compared with the exclusive or mostly formula feeding group (P ranges from <0.05 to <0.001). Higher intensity of lactation (exclusive and mostly breastfeeding groups vs. exclusively or mostly formula feeding) was associated with lower adjusted mean group differences (95% CI) in fasting plasma glucose (mg/dL) by −4.3 (−7.4 to −1.3) and −5.0 (−8.5 to −1.4), respectively (P < 0.01), and fasting insulin (μU/mL) by −6.3 (−10.1 to −2.4) and −7.5 (−11.9 to −3.0), respectively (P < 0.001) (Table 2). Similar associations were found with lower 2-h insulin values of −21.4 (−41.0 to −1.7) for exclusive breastfeeding (P < 0.05) and −36.5 (−59.3 to −13.7) for mostly breastfeeding (P < 0.001) versus exclusive or mostly formula feeding. HOMA-IR was significantly lower and ISI 0, 120 was significantly higher with greater intensity of lactation compared with exclusive or mostly formula feeding women (P < 0.001). No differences in metabolic parameters were found for mixed or inconsistent feeders (similar amounts of breast milk and formula) compared with the formula feeding group. Breastfeeding during the OGTT had no significant impact on the metabolic parameters (data not shown).
Classification as having diabetes (n = 17), prediabetes (n = 171), or normal (n = 334) glucose tolerance based on the 2-h 75-g OGTT was inversely associated with lactation intensity (P = 0.02), with higher prevalence of glucose intolerance as the amount for formula fed increased across the infant feeding groups (Fig. 1A). When stratified by maternal obesity (Fig. 1B), the association between glucose tolerance categories and infant feeding groups remained among the 241 obese women (P = 0.03).
Our large epidemiologic study in a racially and ethnically diverse cohort found a dose-response relationship between increasing intensity of lactation (ranging from exclusive breastfeeding to exclusive or mostly formula feeding) and decreasing fasting plasma glucose and both fasting and 2-h insulin, as well as improved insulin sensitivity at 6–9 weeks’ postpartum. Intensively lactating women displayed 4–5 mg/dL lower mean fasting plasma glucose compared with women who intensively formula fed, but no differences in 2-h glucose. These findings are consistent with the only prior study to examine lactation and metabolic profiles among women with recent GDM. Kjos et al. (4) reported 5 mg/dL lower fasting blood glucose for any intensity of lactation versus no lactation groups in a retrospective cohort of Latinas. However, 2-h glucose levels were 10 mg/dL lower between lactating versus nonlactating groups in this study, but this difference was not observed in our study. Differences in study design, particularly our comparison of intensively lactating women, standardized prenatal screening criteria, and our diverse racial and ethnic groups with lower risk of diabetes, may explain the discrepant findings. The study of Latinas included a longer time period for OGTT screening (up to 12 weeks) and a higher prevalence of postpartum diabetes (6.7 vs. 3% in our study), which may have enhanced differences in the 2-h OGTT results among the two infant feeding groups (lactating vs. nonlactating) reported by Kjos et al. (4).
Our study also enhances our knowledge about the effect of lactation on insulin sensitivity and resistance in women with recent GDM. Our findings support the hypothesis that lactation spares insulin response required for similar or even improved levels of glucose control. Glucose (∼50 g/day) is diverted for lactogenesis (the process of milk synthesis and secretion) via noninsulin-mediated pathways of uptake by the mammary gland (12) and, thus, lower levels of insulin with increasing intensity of lactation is expected. Thus, lactating women exhibit lower blood glucose and insulin concentrations along with higher rates of glucose production and lipolysis compared with nonlactating women (2). In our study, both exclusively and mostly lactating women experienced similar lowering of glucose and insulin levels relative to formula feeding women. The diversion of glucose and lipids into milk production may unload the pancreatic β-cells and preserve long-term insulin production in women. The impact of maternal metabolic profiles on breast milk composition has not been assessed in women with a history of GDM.
Also, we found that indices of insulin sensitivity were associated with higher lactation intensity. The indices were most favorable for exclusive or mostly breastfeeding compared with formula feeding but mixed feeders did not differ from formula feeders. A few small studies (sample size <30) measured insulin and glucose as well as insulin response to any lactation, and results are consistent with our findings. McManus et al. (5) reported improved insulin response and glucose tolerance among lactating women with recent GDM at 3 months’ postpartum. A total of 14 lactating compared with 12 nonlactating women had higher insulin sensitivity, glucose effectiveness, and first-phase insulin response to glucose assessed by Bergman minimal model, but statistical significance was not reached, possibly as a result of the small sample size (5). However, the disposition index (insulin sensitivity multiplied by first-phase insulin response to glucose) was 2.5 times higher (129.9 ± 26.0 vs. 53.4 ± 18.0 × 10(−4) min(−1); P < 0.05) in lactating compared with nonlactating women matched for age, weight, postpartum weight loss, and exercise habits (5). Other small cross-sectional studies report that lactating women with recent GDM had lower insulin levels (3) and insulin-glucose ratios (12) than nonlactating women. In sum, these data suggest improved insulin sensitivity and β-cell function in postpartum women with a history of GDM who breastfeed, but previous studies did not examine intensity of breastfeeding.
Our study strengths include the racially and ethnically diverse sample of women who were diagnosed with GDM via standardized criteria and the detailed and systematic prospective assessment of lactation intensity and duration. There were also some limitations, including the lack of direct measures of insulin sensitivity in women during or after pregnancy and no direct measures of overall or regional adiposity in the cohort based on magnetic resonance imaging or dual X-ray absorptiometry methodologies. BMI is a crude measure of overall adiposity, and we controlled for this potential confounder in the multivariable models as a surrogate marker for healthy lifestyle behaviors that may be associated with infant feeding practices. Because it is unethical to randomize women to breastfeeding or formula feeding, residual confounding may play a role in the improved metabolic control observed with greater lactation intensity. Poorer metabolic status, obesity, or other physiologic traits could influence a woman’s ability to successfully establish lactation (e.g., delayed lactogenesis) and, potentially, the findings may be due to reverse causation. This possibility is minimized because the SWIFT infant feeding groups were comparable in severity of GDM based on the 3-h 100-g OGTT blood glucose results obtained at similar gestational age and the fact that over 50% of obese women in our sample lactated successfully (exclusive or mostly breastfeeding). Our findings are robust given the large sample size of women diagnosed with GDM via standardized methodologies and the more favorable metabolic profiles for lactating women even after adjustment for potential confounders such as prepregnancy BMI, education, race/ethnicity, parity, age, and fasting time period.
Previous evidence consistently shows that women with a history of GDM who lactate manifest improved metabolic profiles as well as enhanced pancreatic β-cell compensation (5) and exhibit a lower prevalence of type 2 diabetes in the early postpartum period (4). Yet direct evidence that links these acute metabolic effects to persistent metabolic changes postweaning or assesses whether they confer protection against future disease onset has been unavailable. Our findings may have important implications for determination of glucose tolerance based on results from the postpartum OGTTs, including the diagnosis of diabetes as well as glucose intolerance (prediabetes). Because intensive lactation results in lower fasting and 2-h glucose levels from the OGTT, it may be advisable to repeat the OGTT postweaning, particularly for women whose glucose values are within 10% below cutoffs for diagnosis of diabetes.
Our findings also support previous evidence that lactation may be beneficial to women’s future health, including protection against development of metabolic diseases in midlife. The Coronary Artery Risk Development in Young Adults (CARDIA) study reports that longer duration of lactation (>1–5 months, >5–9 months, and >9 months vs. 0–1 month) was associated with lower incidence of metabolic syndrome in midlife (lower by 39–50% for non-GDM and by 49–86% among GDM groups independent of preconception metabolic profiles, weight gain, sociodemographics, and lifestyle behaviors) among black women, and white women followed prospectively (13). Other studies report that lactation had weak to modest effects in reducing metabolic diseases or cardiovascular risk factors (14–18). Only one study examined lactation duration and incident diabetes among women with a history of GDM, but it reports a null association based on the retrospective design (18). Thus, benefits of lactation for prevention of diabetes are likely, but direct evidence is much less available in women with previous GDM. Moreover, none of the previous studies ever examined lactation intensity. The findings from the CARDIA study by Gunderson et al. (13) are unique because of the repeated measures longitudinal design that involved prospective data collection every 2–5 years (i.e., lactation duration, changes in lifestyle behaviors, body weight, and biochemical measures during the reproductive years), as well as control for prepregnancy metabolic syndrome components. Lactation duration beyond 6 months may be a proxy for higher intensity. Our study findings support the hypothesis that exclusive lactation for shorter time periods may confer additional long-term health benefits for women, particularly women with GDM who are up to seven times more likely to develop diabetes in midlife (19,20).
Postpartum screening for diabetes after GDM pregnancy is completed by only ∼50% of women with GDM in various clinical settings (21), even though both the American Diabetes Association and the American College of Obstetrics and Gynecology recommend screening for early diagnosis and treatment of diabetes after pregnancy (22,23). Future studies are needed to better understand the mechanisms through which lactation may contribute to lasting improvements in metabolic profiles and the potential degree of benefit to long-term health according to various levels of intensity and duration of lactation.
This study was funded by grants from the National Institute of Child Health and Human Development (R01-HD-050625, R01-HD-050625-03S1, and R01-HD-050625-05S). This project was also supported in part by the National Institutes of Health National Center for Research Resources UCSF-CTSI UL1 RR024131.
No potential conflicts of interest relevant to this article were reported.
E.P.G. designed the study, collected data, and wrote the manuscript. M.M.H. contributed to data collection and edited the manuscript. V.C. conducted the data analysis and developed the tracking and data collection programs. Y.C. provided consultation regarding patient recruitment and supported participant recruitment from the Santa Clara field site. D.W. provided consultation regarding patient recruitment and supported participant recruitment from the Oakland field site. R.A.A. provided consultation regarding patient recruitment and supported participant recruitment from the Roseville and Sacramento field sites. G.F. provided consultation regarding patient recruitment and supported participant recruitment from the South Sacramento field site. C.E. provided consultation regarding patient recruitment and supported participant recruitment from the North Valley field sites. S.Y. provided consultation regarding patient recruitment and supported participant recruitment from the Fremont and Hayward field sites. N.S. provided consultation regarding patient recruitment and supported participant recruitment from the Richmond field site. M.L. provided consultation regarding patient recruitment and supported participant recruitment from the San Jose field site. C.P.Q. provided biostatistical consultation and data interpretation. J.C.L. provided consultation for screening via the 2-h OGTT and edited the manuscript. B.S. assisted in the development of behavioral assessment tools and edited the manuscript. A.F. assisted in methodologies for GDM diagnosis from electronic data and edited the manuscript. J.V.S. provided study design consultation and supported participant recruitment from Kaiser Permanente Northern California regionwide and at the Division of Research clinic.
The contents of this article are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health.
- Received July 26, 2011.
- Accepted September 13, 2011.
- © 2012 by the American Diabetes Association.
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