Immunologic Properties of Biosynthetic Human Insulin in Vitro
- Address reprint requests to W. Beischer, Department of Internal Medicine I, University of Ulm, Steinhövelstr. 9, D-7900, Ulm, Germany.
Abstract
Some immunologic properties of biosynthetic human insulin (BHI) were examined in vitro. An identical behavior was found for BHI and pancreatic human insulin as standard preparation and for A14-mono-labeled BHI and pork insulin as tracer in the insulin radioimmunoassay. BHI proved to be free of human proinsulin and C-peptide. Insulin antibodies in serum of two diabetic patients showed a preferential binding of 125I-bovine insulin. However, the antibody titers were almost identical for A14-mono-labeled BHI and pork insulin. These studies did not reveal any characteristic immunologic properties of BHI compared with highly purified pancreatic human and pork insulin.
- Copyright © 1981 by the American Diabetes Association
