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Short Reports

Superiority of Radiobinding Assay Over ELISA for Detection of IAAs in Newly Diagnosed Type I Diabetic Children

  1. Claire Levy-Marchal, MD,
  2. Marie-Pascale Bridel,
  3. Françoise Sodoyez-Goffaux, MD,
  4. Michel Koch, PhD,
  5. Jean Tichet, MD,
  6. Paul Czernichow, MD and
  7. Jean-Claude Sodoyez, MD
  1. Departments of Diabetology and Biochemistry, Robert Debré Hospital Paris; and the Regional Institute of Health Tours, France; and the Departments of Pediatrics and Endocrinology, Sart-Tilman University Liège, Belgium
  1. Address correspondence and reprint requests to Claire Levy-Marchal, MD, Service de Diabeétologie, Hôpital Robert Debré, 48 Boulevard Séurier, 75019 Paris, France.
Diabetes Care 1991 Jan; 14(1): 61-63. https://doi.org/10.2337/diacare.14.1.61
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Abstract

Objective Liquid- or solid-phase assays have been used for insulin autoantibody (IAA) determination, and the method of IAA measurement has not been standardized.

Research Design and Methods IAAs were determined by radiobinding assay (RBA) and enzyme-linked immunosorbent assay (ELISA) in two large age-matched groups of nondiabetic and newly diagnosed insulin-dependent (type I) diabetic children.

Results Positivity for IAA by RBA (≥ to nondiabetic mean + 3SD) was 2 of 178 (1.1%) and 55 of 173 (32%) in nondiabetic and diabetic children, respectively. Prevalence of IAA by RBA was significantly higher in the youngest age-group (63% between 0-4 yr). Positivity for IAA by ELISA was 1 of 178 (0.6%) and 8 of 169 (4.7%) in nondiabetic and diabetic children, respectively. Concordance rates between both assays were 0 of 3 (0%) in control subjects and 5 of 58 (8.6%) in diabetic children.

Conclusions We conclude that RBA is more appropriate than ELISA for IAA detection at the onset of the disease. In addition, because available data suggest that IAAs detected by RBA only are high-affinity antibodies, it is tempting to speculate that IAAs reflect a mature immune reaction against endogenous insulin.

  • Received April 16, 1990.
  • Revision received July 27, 1990.
  • Accepted July 27, 1990.
  • Copyright © 1991 by the American Diabetes Association
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Superiority of Radiobinding Assay Over ELISA for Detection of IAAs in Newly Diagnosed Type I Diabetic Children
Claire Levy-Marchal, Marie-Pascale Bridel, Françoise Sodoyez-Goffaux, Michel Koch, Jean Tichet, Paul Czernichow, Jean-Claude Sodoyez
Diabetes Care Jan 1991, 14 (1) 61-63; DOI: 10.2337/diacare.14.1.61

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Superiority of Radiobinding Assay Over ELISA for Detection of IAAs in Newly Diagnosed Type I Diabetic Children
Claire Levy-Marchal, Marie-Pascale Bridel, Françoise Sodoyez-Goffaux, Michel Koch, Jean Tichet, Paul Czernichow, Jean-Claude Sodoyez
Diabetes Care Jan 1991, 14 (1) 61-63; DOI: 10.2337/diacare.14.1.61
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