Relationship Between Absorption of Radiolabeled Soluble Insulin, Subcutaneous Blood Flow, and Anthropometry

Jiten P Vora; Anna Burch; John R Peters; David R Owens

doi:10.2337/diacare.15.11.1484

Original Articles

Relationship Between Absorption of Radiolabeled Soluble Insulin, Subcutaneous Blood Flow, and Anthropometry

Jiten P Vora, MD,
Anna Burch, MPH,
John R Peters, FRCP and
David R Owens, MD

Departments of Medicine and Medical Physics, University of Wales College of Medicine Heath Park, Cardiff, United Kingdom

Address correspondence and reprint requests to Jiten P. Vora, MD, Division of Nephrology and Hypertension, Oregon Health Sciences University, 3181 Sw Sam Jackson Park Avenue, Portland, OR 97201.

Diabetes Care 1992 Nov; 15(11): 1484-1493. https://doi.org/10.2337/diacare.15.11.1484

Abstract

OBJECTIVE To evaluate the interrelationships between the rate of absorption of soluble insulin, SCBF, and anthropometry in normal subjects.

RESEARCH DESIGN AND METHODS In 12 normal men (age range 23–30 yr, BMI 18.2–41.3 kg/m²), simultaneous assessment of the absorption of ¹²⁵I-labeled soluble insulin and SCBF (^99mTc clearance) was performed, on separate study days, for the anterior abdominal wall, anterior midthigh, and the upper arm sites. Each site was examined in a randomized order on two separate occasions. Absorption of ¹²⁵I-soluble insulin was determined by external monitoring of residual radioactivity levels at the injection site for 6 h postinjection. Residual radioactivity level-time curves, including the characteristic early phase of slow absorption of soluble insulin (the lag phase), were described using two- and three-parameter biexponential models. Anthropometric measurements included BMI, ultrasonic measurement of the subcutaneous adipose tissue layer, and caliper skin fold thickness at the anterior abdominal wall, biceps, triceps, anterior midthigh, and subscapular sites.

RESULTS A highly significant positive relationship was observed between the rate of absorption of ¹²⁵I-soluble insulin and SCBF (r_S =0.44–0.52; P < 0.01–0.001). The duration of the lag phase was inversely correlated with SCBF (r_S = −0.34 – −0.51; P < 0.01–0.001). Inverse relationships also were observed for the subjects' degree of adiposity with the rate of soluble insulin absorption (r_s = −0.43–−0.71; P < 0.001) and SCBF (r_S = −0.27–−0.62; P < 0.05–0.001). Significantly shorter lag phase was observed for the abdominal site compared with thigh and arm injection sites (P < 0.05–0.01).

CONCLUSIONS The rate of absorption of soluble insulin, including during the lag phase, is positively correlated with SCBF. Increasing adiposity prolongs the duration of the early lag phase and reduces the rate of absorption of soluble insulin and SCBF.