Internalization of 125I-Insulin by IM-9 Cultured Human Lymphocytes: A Comparison Between A14-Monoiodo-Pork and Biosynthetic Human Insulin

Jean Louis Carpentier; Philippe A Halban; Albert E Renold; Lelio Orci

doi:10.2337/diacare.4.2.220

Original Articles

Internalization of ¹²⁵I-Insulin by IM-9 Cultured Human Lymphocytes: A Comparison Between A14-Monoiodo-Pork and Biosynthetic Human Insulin

Jean Louis Carpentier,
Philippe A Halban,
Albert E Renold and
Lelio Orci

Institute of Histology and Embryology, University of Geneva School of Medicine Geneva, Switzerland
Institute of Clinical Biochemistry, University of Geneva Geneva, Switzerland

Address reprint requests to J.-L. Carpentier, The Institute of Histology and Embryology, University of Geneva School of Medicine, 1211 Geneva 4, Switzerland.

Diabetes Care 1981 Mar; 4(2): 220-222. https://doi.org/10.2337/diacare.4.2.220

Abstract

Human insulin synthesized from A- and B-chains separately produced in Escherichia coli from cloned genes was characterized by examining its interaction biochemically and morphologically with IM-9 cultured human lymphocytes. Biosynthetic human insulin (BHI) behaves similarly to pork insulin with respect both to its binding properties and to the rate and magnitude at which it is internalized by the cells.