Enzymatic Glucose Electrodes

George G Guilbault

doi:10.2337/diacare.5.3.181

Symposium on Potentially Implantable Glucose Sensors

Enzymatic Glucose Electrodes

George G Guilbault

Department of Chemistry, University of New Orleans New Orleans, Louisiana

Address reprint requests to George G. Guilbault, Professor of Chemistry, University of New Orleans, Lake Front, Department of Chemistry, New Orleans, Louisiana 70122.

Diabetes Care 1982 May; 5(3): 181-183. https://doi.org/10.2337/diacare.5.3.181

Abstract

Enzymatic electrodes are described for the specific and sensitive determination of glucose in blood. The probes are based on the conversion of B-D-glucose by glucose oxidase chemically attached to the surface of a Pt electrode. The peroxide liberated is sensed by measurement of the current generated at +0.7 volts vs. S.C.E. The enzyme, chemically attached to pig intestine via glutaraldehyde coupling is quite stable for months, even at room temperature. Other glucose electrodes developed at the University of New Orleans, using Pt (measurement of the O₂ consumed at −0.7 volts vs. S.C.E.) and I⁻ probes are also described.