Table 1—

Characteristics of the islet allograft

Patient and infusion procedure numberAge of donor (years)Donor’s BMI (kg/m2)Cause of donor deathDuration of cold ischemia (hours) from cross clamping to islet isolationTotal islet infused (IEQ)Pellet volume (cc) (pure fraction/less pure fraction)*Purity % (pure fraction/less pure fraction)Islet viability % (pure fraction/less pure fraction)
Patient 1
 14328.4Intracerebral hemorrhage5.1276,3331.58591 ± 10
2.06591 ± 17
 25330.2Intracerebral hemorrhage6.5316,9332.37087 ± 13
2.04090 ± 10
Patient 2
 15923.7Stroke3.2337,8480.87090 ± 19
7.03587 ± 17
Patient 3
 16426.6Intracerebral hemorrhage2.6244,5802.09079 ± 33
1.54091 ± 9
 23434.0Trauma6.3316,1602.07081 ± 27
3.03085 ± 25
Patient 4
 15329.7Intracerebral hemorrhage7.2317,2721.57084 ± 25
2.56077 ± 30
 23932.3Intracerebral hemorrhage4.8509,0002.09087 ± 13
3.04587 ± 20
Patient 5
 16119.6Intracerebral hemorrhage3.25298,5002.08591 ± 12
3.03091 ± 15
 24734.0Intracerebral hemorrhage6361,0003.07587 ± 16
Patient 6
 14127.7Trauma7.5435,4823.08087 ± 18
  • * The calculated volume of 300,000 efficiently packed IEQs is ∼1 cm3. One can thus double-check microscopically determined IEQ counts by using the packed cell volume and the purity assessment. For instance, the islet preparation for patient #6 was estimated to contain, by sampling and physical counting, 435,482 islets. The islet cell pellet was measured as being ∼3.0 ml (or cm3) with an ∼80% purity, and as such another way of estimating that recipient’s islet dose would be (3.0 ml × 300,000 IEQ/ml × 80%) or 720,000 IEQ. Islet isolation experts recognize the considerable inaccuracy inherent when calculating IEQs and by convention have used the manual count IEQ determination. The higher numbers calculated by cell pellet volume and estimated islet purity are thought to reflect an overestimation of visually determined islet purity.

  • Due to abdominal pain and increased portal pressure, only 270,000 islets were infused.