Table 1—

Expression of MMPs, TIMPs, cytokines, and connexins in skin biopsies and cultured keratinocytes

DiabeticControlP
MMP-1
    Skin biopsy13.4 ± 1.611.8 ± 2.6NS
    Cultured keratinocytes5.6 ± 1.07.7 ± 3.7NS
MMP-2
    Skin biopsy0.8 ± 0.63.4 ± 3.3<0.05
    Cultured keratinocytes5.8 ± 0.46.6 ± 3.6NS
MMP-9
    Skin biopsy7.7 ± 0.97.0 ± 0.6NS
    Cultured keratinocytes11.7 ± 1.78.8 ± 0.4NS
TIMP-1
    Skin biopsy2.7 ± 0.73.5 ± 2.8NS
    Cultured keratinocytes3.4 ± 0.73.2 ± 0.9NS
TIMP-2
    Skin biopsy0.5 ± 0.41.6 ± 2.3NS
    Cultured keratinocytes5.5 ± 1.65.6 ± 2.7n.s
MMP2/TIMP2 7 h after transplantation1.2 ± 0.31.8 ± 0.4<0.01
IL-β
    Skin biopsy8.6 ± 1.310.4 ± 1.2<0.05
    Cultured keratinocytes4.6 ± 1.03.0 ± 1.4<0.05
TNF-α
    Skin biopsy5.3 ± 0.77.3 ± 2.0<0.05
    Cultured keratinocytes11.0 ± 3.711.0 ± 3.5NS
Cx26
    Skin biopsy3.3 ± 0.73.5 ± 1.6NS
    Cultured keratinocytes2.0 ± 0.32.0 ± 0.9NS
Cx43
    Skin biopsy0.5 ± 0.40.3 ± 0.2NS
    Cultured keratinocytes4.1 ± 1.03.1 ± 1.2NS
  • Data are means ± SD. Units are ΔCt values between the various MMPs, TIMPs, cytokines, and connexins and GAPDH. NS, not significant.